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fluoroshield mounting medium containing 4,6- diamidino- 2- phenylindole (dapi  (Millipore)


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    Structured Review

    Millipore fluoroshield mounting medium containing 4,6- diamidino- 2- phenylindole (dapi
    Fluoroshield Mounting Medium Containing 4,6 Diamidino 2 Phenylindole (Dapi, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluoroshield+mounting+medium+containing+dapi/fluoroshield+dapi/pm38952076-90-5-9
    Average 90 stars, based on 1 article reviews
    fluoroshield mounting medium containing 4,6- diamidino- 2- phenylindole (dapi - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Staining:

    Article Title: Pathogenic Stress Induces Human Monocyte to Express an Extracellular Web of Tunneling Nanotubes
    Article Snippet: Secondary antibodies (Cy3-conjugated AffiniPure F(ab') 2 Fragment Goat anti-Mouse IgG (Jackson ImmunoResearch) or Goat anti-Mouse IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 (ThermoFisher Scientific)) were diluted 1:200 in 2% BSA/PBS and added for 20 min at RT. .. For staining of nuclei and stabilization of fluorescent signals the samples were covered with Fluoroshield mounting medium containing DAPI (F6057, Sigma). .. Cells were visualized by Nikon Ti2E confocal fluorescent microscope with Yokogawa W1 Spinning Disk integrated with 50 μm pinhole, at 580 and 647 nm using a 60x-0.95NA CFA Plan-Apochromat Lambda objective.

    Article Title: Liver receptor homolog-1 (NR5A2) orchestrates hepatic inflammation and TNF-induced cell death.
    Article Snippet: Blocking of unspecific binding took place in blocking solution (3% BSA, 5% goat serum in 0.1% Saponin in PBS) for 60 min, whereafter cells were stained with 1:500 rabbit anti-RelA/p65 (Cell Signaling Technologies) in blocking solution overnight. .. After three times 5 min washing in PBS, cells were stained with 1:500 goat anti-rabbit Alexa Fluor 568 secondary antibody for 1.5 h. Following three times washing, cells were covered with Fluoroshield mounting medium containing DAPI (Sigma) to stain nuclei and mount samples. ..

    Article Title: Antimicrobial peptide LL-37 and its pro-form, hCAP18, in desquamated epithelial cells of human whole saliva.
    Article Snippet: The antimicrobial peptide LL-37 is active against oral bacteria and has been demonstrated to be present in human saliva, but its distribution in different fractions of saliva is not known.. LL-37 is formed from its intracellular pro-form, hCAP18, in an extracellular enzymatic reaction catalyzed by proteinase 3 and kallikrein 5.. Here, we prepared cell-containing and cell-free fractions of unstimulated human whole saliva by centrifugation after depolymerization of mucins with dithiothreitol, and measured the levels of hCAP18/LL-37 in these fractions using ELISA.

    Article Title: Inhibition of HDAC6 Attenuates Diabetes-Induced Retinal Redox Imbalance and Microangiopathy
    Article Snippet: .. Stained cells were then washed and suspended in HBSS, mounted using Fluoroshield mounting medium containing DAPI (Sigma-Aldrich) and immediately analyzed under a Zeiss Axioplan-2 fluorescence microscope (Carl Zeiss). .. To evidence senescent HuREC, we used senescence-associated β-Galactosidase (SA-β-Gal) reactivity-based assay using a commercially available kit (Cell Signaling) as previously shown [ ].

    Fluorescence:

    Article Title: Inhibition of HDAC6 Attenuates Diabetes-Induced Retinal Redox Imbalance and Microangiopathy
    Article Snippet: .. Stained cells were then washed and suspended in HBSS, mounted using Fluoroshield mounting medium containing DAPI (Sigma-Aldrich) and immediately analyzed under a Zeiss Axioplan-2 fluorescence microscope (Carl Zeiss). .. To evidence senescent HuREC, we used senescence-associated β-Galactosidase (SA-β-Gal) reactivity-based assay using a commercially available kit (Cell Signaling) as previously shown [ ].

    Microscopy:

    Article Title: Inhibition of HDAC6 Attenuates Diabetes-Induced Retinal Redox Imbalance and Microangiopathy
    Article Snippet: .. Stained cells were then washed and suspended in HBSS, mounted using Fluoroshield mounting medium containing DAPI (Sigma-Aldrich) and immediately analyzed under a Zeiss Axioplan-2 fluorescence microscope (Carl Zeiss). .. To evidence senescent HuREC, we used senescence-associated β-Galactosidase (SA-β-Gal) reactivity-based assay using a commercially available kit (Cell Signaling) as previously shown [ ].



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